Cloning of a serotype-specific antigen from Pasteurella haemolytica A1
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منابع مشابه
Cloning of a serotype-specific antigen from Pasteurella haemolytica A1.
Recombinant plasmids coding for a soluble (or surface) antigen of Pasteurella haemolytica A1 were identified. Two plasmids, both containing the same 5.4 kilobase pairs of insert DNA, were recovered independently by screening a clone band of P. haemolytica A1 genomic DNA in Escherichia coli for the expression of P. haemolytica A1 soluble antigens (R. Y. C. Lo and L.A. Cameron, Can. J. Biochem. C...
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A Pasteurella haemolytica A1 gene involved in the biosynthesis of a moiety on the core of the lipopolysaccharide molecule has been cloned and characterized. Escherichia coli clones which carry this gene showed an alteration of its lipopolysaccharide migration profile on tricine SDS-PAGE and exhibited resistance to the core-specific phage U3. In addition, lipopolysaccharide extracted from the E....
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Mice immunized with the potassium thiocyanate extract of Pasteurella haemolytica, serotype 1, were found to resist a challenge infection of P. multocida type A, thus demonstrating cross-protection. This finding was further supported by the finding that an antiserum directed against the potassium thiocyanate extract of P. haemolytica was bactericidal to P. multocida and vice versa.
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In order to show the type and severity of gross and histopathologic lesions induce by vaccinal strain (serotype A1) of Pateurella mulocida, ten four-week-old SPF chickens were inoculated intramuscularly with 75 cfu of ( 0.5 ml of 10-7 dilution) bacterium. All birds died in less than 16 hours. No prominent gross lesions were observed in different organs. In microscopic examination, the most comm...
متن کاملVirulence of Avian Serotype A1 Pasteurella multocida for Chickens and Mice.
The virulence of Pasteurella multocida (P. multocida) serotype A1 for chickens and mice was determined. Groups of chicken and mice were exposed intramuscularly and intraperitoneally to various concentration of P. multocida broth culture, respectively. This strain was highly virulent for chickens so that those exposed to only 7 c.f.u. of the organism died in less than 24 hours. Groups of mice ex...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1986
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.53.3.505-510.1986